Polymorphism of ESR and DGKH DNA markers in Edilbay and Cygai sheep

The polymorphism of DNA markers of diacylglycerol kinase eta (DGKH) and estrogen receptor (ESR) was studied in ram lambs of the Cygai (n=150) and Edilbay (n=135) breeds. Genotyping revealed the presence of CC, CT, and TT genotypes for the DGKH gene in the Cygai breed, while the CC genotype was not detected in the Edilbay breed. For the ESR gene, CC, TC, and TT genotypes were identified in both breeds, with differences in their distribution: in the Cygai breed, the distribution was more balanced, whereas in the Edilbay breed the TT genotype predominated. Analysis of the data showed a higher level of genetic diversity for the ESR gene in the Cygai breed and conformity to Hardy–Weinberg equilibrium, while in the Edilbay breed the DGKH gene demonstrated a statistically significant deviation from equilibrium due to an excess of heterozygotes. The results indicate breed-specific differences in allele and genotype distribution for the studied markers.

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