Development and validation of a test system for sequencing a fragment of the PRNP gene of domestic goats
https://doi.org/10.26897/2074-0840-2024-2-3-8
Abstract
The article presents the stages of development of a universal test system to establish the genotype in the codons of the target fragment of exon 3 of the prion protein (PRNP) gene in domestic goats (Capra hircus). The test system has been tested. The presence of polymorphism in the target fragment of exon 3 of the PRNP gene in domestic goats was revealed. In the 20 goat samples, various allelic variants were identified at codons 37, 40, 122, 142, 143, 146, 154, 211, 222, 240 and 244, respectively. The developed test system will allow us to assess the level of polymorphism in the target fragment of the PRNP gene in domestic goats of various breeds raised in the Russian Federation.
Supporting agencies: The work was carried out with the support of the Ministry of Science and Higher Education of the Russian Federation (topic FGGN-2024-0015).
About the Authors
N. F. Bakoev
Federal Research Center of Animal Husbandry - VIZ named after Academician L.K. Ernst
Russian Federation
Russian Federation
Nekruz F. Bakoev - Candidate of Agricultural Sciences, sci. employee of the Laboratory of Population and Evolutionary Genomics of animals.
Moscow region, Podolsk city district, Dubrovitsy village
Tel.: (977) 106-19-22
T. E. Deniskova
Federal Research Center of Animal Husbandry - VIZ named after Academician L.K. Ernst
Russian Federation
Russian Federation
Tatyana E. Deniskova - Ph D. Biol. sciences, Associate Professor, ved. sci. employee of the Small cattle Genetics and Genomics Group.
Moscow region, Podolsk city district, Dubrovitsy village
Tel.: (916) 914-20-17
O. A. Koshkina
Federal Research Center of Animal Husbandry - VIZ named after Academician L.K. Ernst
Russian Federation
Russian Federation
Olga A. Koshkina - a scientist. Member of the Small Cattle Genetics and Genomics Group.
Moscow region, Podolsk city district, Dubrovitsy village
Tel.: (926) 532-21-19
S. N. Petrov
Federal Research Center of Animal Husbandry - VIZ named after Academician L.K. Ernst
Russian Federation
Russian Federation
Sergey N. Petrov - Ph D. Biol. sciences, art. scientific. employee of the Laboratory of Population and Evolutionary Genomics of animals.
Moscow region, Podolsk city district, Dubrovitsy village
Tel.: (926) 270-97-81
N. A. Zinovieva
Federal Research Center of Animal Husbandry - VIZ named after Academician L.K. Ernst
Russian Federation
Russian Federation
Natalia A. Zinovieva - Doctor of Biology, Professor, Academician of the Russian Academy of Sciences, Director.
Moscow region, Podolsk city district, Dubrovitsy village
Tel.: (4967) 65-11-63
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Review
For citations:
Bakoev N.F., Deniskova T.E., Koshkina O.A., Petrov S.N., Zinovieva N.A. Development and validation of a test system for sequencing a fragment of the PRNP gene of domestic goats. Sheep, goats, wool business. 2024;(2):3-8. (In Russ.) https://doi.org/10.26897/2074-0840-2024-2-3-8
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